Fig. 2
A delayed induction of IFNB response hampers the capacity of PCNs to stop ZIKV replication. PCNs from B6 embryos, non-infected (NI), non-treated (NT) or treated with anti-IFNAR antibodies or recIFNB before infection, were mock- or ZIKV-infected at a MOI of 5. a–d Neutralization of type-I IFN response with anti-IFNAR antibodies has no significant effect on ZIKV replication in PCNs until 72 h p.i. while treatment with recIFNB inhibits ZIKV replication from the onset of infection as shown by the relative expression of RNA levels analyzed by RT-qPCR with respect to Rplp0 used as reference gene. Symbols represent values obtained from n = 5 independent experiments with varying conditions analyzed in each experiment with a minimum of two different times p.i. analyzed per condition per experiment. a–c Bars represent means without s.d. with significance assessed in a by two-way ANOVA and Tukey’s multiple comparisons test. d Means are represented in graph. e PCNs from B6 embryos ZIKV-infected at a MOI of 5, either non-treated (NT) or treated with recIFNB added before infection (early recIFNB) or 48 h p.i. (late recIFNB), were collected 64 h p.i.. Results correspond to those obtained from n = 3 independent PCNs shown in different colors. Means are represented in graph with significance assessed by paired t-test. Only early recIFNB significantly inhibited ZIKV RNA production measured by RT-qPCR with respect to Rplp0 as reference gene. f MEFs from three different B6 embryos, either non-treated (NT) or treated with anti-IFNAR antibodies, were mock- or ZIKV-infected at a MOI of 5. Neutralization of type-I IFN response with anti-IFNAR antibodies significantly affected ZIKV starting at 48 h p.i. as shown by the relative expression of ZIKV RNA levels analyzed by RT-qPCR with respect to Rplp0 used as reference gene. Symbols represent values obtained from each of the n = 3 different embryos. Bars represent means without s.d. with significance assessed in by two-way ANOVA and Tukey’s multiple comparisons test. P-value < 0.001 (***), < 0.01 (**), < 0.05 (*) and ns not significant