Fig. 4

YTHDF1 regulates morphine-induced inflammation in the vlPAG. Knockdown of YTHDF1 in the vlPAG by CMV-YTHDF1-shRNA in WT mice inhibited the expression (IHC) of inflammatory factors such as IL-1β (a), IL-6 (c) TNF-α (e), and NF-κB (g). Scale bars, 50 μm. b, d, f, h Knockdown of YTHDF1 in the vlPAG by shRNA in virus-infected WT mice inhibited the expression (western blotting) of inflammatory factors such as IL-1β (b, ***P < 0.001), IL-6 (d, **P = 0.00612), TNF-α (f, **P = 0.00209) and NF-κB (h, **P = 0.0023) (n = 6, Student’s t test, two-tailed. Data are shown as the mean ± SEM; CMV-YTHDF1-shRNA-NC = AAV-CMV-Cre + AAV-CMV-DIO-EGFP-YTHDF1-shRNA-NC; CMV-YTHDF1-shRNA = AAV-CMV-Cre + AAV-CMV-DIO-EGFP-YTHDF1-shRNA)