Fig. 5

YTHDF1 regulates morphine-induced inflammation in the vlPAG. Knockdown of YTHDF1 in the vlPAG by CMV-Cre-EGFP in YTHDF1^fl/fl mice inhibited the expression (IHC) of inflammatory factors such as IL-1β (a), IL-6 (c) TNF-α (e), and NF-κB (g). Scale bars, 50 μm. b, d, f, h Knockdown of YTHDF1 in the vlPAG by CMV-Cre-EGFP in YTHDF1^fl/fl mice inhibited the expression (western blotting) of inflammatory factors such as IL-1β (b, ***P < 0.001), IL-6 (d, **P = 0.00202), TNF-α (f, **P = 0.00749) and NF-κB (h, ***P < 0.001) (n = 6), Student’s t test, two-tailed. Data are shown as the mean ± SEM