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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Delayed NLRP3 inflammasome inhibition ameliorates subacute stroke progression in mice

Fig. 3

MCC950 has an enhanced long-term neuroprotective effect and reduces reactive astrogliosis after prophylactic and delayed application. A Ratio of ipsilesional to contralesional NeuN intensity of coronary brain slices 7 days after tMCAO of vehicle-, MCC950 d0- and MCC950 d1-treated mice. Three sections per animal. (n ≥ 7). B Number of NeuN positive cells per 0.1 mm2 counted in 10 corresponding regions on three sections per animal 7 days after tMCAO of vehicle-, MCC950 d0- and MCC950 d1-treated mice as indicated in (C). D Representative immunohistochemical stainings of NeuN (neurons, green) of coronary brain slices 7 d after tMCAO in vehicle-, MCC950 d0- and MCC950 d1-treated mice using 5 × objective. Scale bar = 2 mm. Infarct areas highlighted by a grey line. The white rectangles mark areas at the border of infarct core and penumbra as magnified 20-fold in (E). 20 × objective. Scale bar = 100 µm. F GFAP protein content in cortex or basal ganglia of vehicle-, MCC950 d0- and MCC950 d1-treated mice. For densitometric quantification actin was used as a loading control (n ≥ 7). G Representative penumbral stainings for GFAP of vehicle-, MCC950 d0- and MCC950 d1-treated mice. Scale bar = 50 µm. For densitometric quantification actin was used as a loading control. Data were analyzed by one-way ANOVA with post hoc Tukey adjustment for p values. *p < 0.05; **p < 0.01; ***p < 0.001

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