Fig. 4

Prophylactic and delayed NLRP3 inhibition prevent long-range immune cell infiltration after IS. A Left: quantification of brain-infiltrating CD4-positive cells of vehicle-, MCC950 d0- and MCC950 d1-treated mice within 5 ipsilesional and 5 contralesional priorly defined segments as depicted in the center. Right: representative immunocytologic stainings (localization marked with a *) of CD4-positive lymphocytes (red) and nuclei (DAPI, blue) in the ipsilateral hemisphere on day 7 after tMCAO in vehicle-, MCC950 d0- and MCC950 d1-treated mice using 20 × objective. Scale bar = 20 μm (n ≥ 7). B Left: quantification of Iba1-positive cells of vehicle-, MCC950 d0- and MCC950 d1-treated mice within 5 ipsilesional and 5 contralesional priorly defined segments as depicted in the center. Right: representative immunocytologic stainings (localization marked with a *) of Iba1-positive microglia (green) and nuclei (DAPI, blue) in the ipsilateral hemisphere on day 7 after tMCAO in vehicle-, MCC950 d0- and MCC950 d1-treated mice using 20 × objective. Scale bar = 20 μm (n ≥ 7). C Left: quantification of brain-infiltrating Ly.6 g-positive cells of vehicle-, MCC950 d0- and MCC950 d1-treated treated mice within 5 ipsilesional and 5 contralesional priorly defined segments as depicted in the center. Right: representative stainings (localization marked with a *) of Ly.6 g-positive neutrophils in the ipsilateral hemisphere on day 7 after transient middle cerebral artery occlusion (tMCAO) in vehicle-, MCC950 d0- and MCC950 d1-treated mice using 10 × objective. Scale bar = 100 μm (n ≥ 7). Data were analyzed by one-way ANOVA with post hoc Tukey adjustment for p values. *p < 0.05, **p < 0.01; ***p < 0.001