Skip to main content
Fig. 9 | Journal of Neuroinflammation

Fig. 9

From: Fecal microbiota transplantation and replenishment of short-chain fatty acids protect against chronic cerebral hypoperfusion-induced colonic dysfunction by regulating gut microbiota, differentiation of Th17 cells, and mitochondrial energy metabolism

Fig. 9

FMT and SCFAs treatment relieve CCH-induced colonic mitochondrial dysfunction. A The activities of electron transport chain complex I-V in colon (n = 5 per group). B The content of ATP in colon (n = 5 per group). C The content of acetyl coenzyme A in colon (n = 5 per group). D Representative DHE fluorescence staining for ROS and relative level of ROS in colon (ratio of sham group, n = 3 per group, scale bars = 20 μm). The average area of ROS-positive puncta in sham group is set to 1. E Representative electron micrographs of mitochondria in colon (scale bars = 0.5 µm). F Representative western blot for ND4,COX1 and VDAC1 in colon. G Relative optical density analysis for ND4,COX1 and VDAC1 in colon (n = 4 per group). H Representative JC-1 fluorescence staining for mitochondrial membrane potential (scale bars = 50 µm). Impaired mitochondria: low intensity of red fluorescence but high intensity of green fluorescence. Healthy mitochondria: high intensity of red fluorescence but low intensity of green fluorescence. Data are expressed as mean ± SD. *P < 0.05 vs. sham group. #P < 0.05 vs. BCCAo group

Back to article page

Journal of Neuroinflammation

ISSN: 1742-2094