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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: Inhibition of CCR2 attenuates neuroinflammation and neuronal apoptosis after subarachnoid hemorrhage through the PI3K/Akt pathway

Fig. 4

CCR2 increased cell viability and inhibited hemin-induced cell inflammation and apoptosis at 24 h after hemin exposure. A Cell viability decreased with increasing hemin concentration (n = 6). B CCK-8 results showed that the cell viability decreased significantly after treatment with hemin (160 mmol/L). RS (10 μmol/L; n = 6) rescued the process. C, D Representative western blot strips and CCR2 density quantified the efficacy of RS on hemin-induced SAH, n = 3 per group. E–I Representative western blot bands and densitometric quantification of Blc-2, Bax, IL-1β and TNF-α in hemin-induced SAH in vitro, n = 6 per group. J, K ELISA detected the concentrations of IL-1β and TNF-α in cell lysates after homogenization. L, M Representative flow cytometry images of HT22 cells, apoptotic cells represented by 7-AAD+/Annexin V+ ratio. *P < 0.05 vs Sham group. **P < 0.01 vs Sham group. #P < 0.05 vs SAH group. ##P < 0.01 vs SAH group

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