Fig. 6

LY abolished the anti-neuroinflammation and anti-apoptosis effects of RS at 24 h in vitro. A Representative western blot bands. B–H Densitometric quantification of CCR2, p-PI3K, PI3K, p-AKT, AKT, Bcl-2, Bax, and CC3, IL-1β and TNF-α at 24 after hemin-induced SAH. I, J ELISA detected the concentrations of IL-1β and TNF-α in cell lysates after homogenization. K, L Representative flow cytometry images of HT22 cells, apoptotic cells represented by 7-AAD+/Annexin V+ ratio. *P < 0.05 vs Sham group; **P < 0.01 vs Sham group. #P < 0.05 vs Hemin group. ^##P < 0.01 vs Hemin group; ^&P < 0.05 vs. Hemin + RS group; ^&&P < 0.01 vs. Hemin + RS group; n = 6 per group