Fig. 1

Hierarchical clustering and significance analysis of microarrays. A Heatmap of a representative GSE40857 dataset shows a dendrogram of genes to the left of the figure, which includes the 8617 most differentially expressed genes, before false discovery rate (FDR) correction. The dendrogram of samples at the top shows almost all the control samples grouping in one cluster (light grey bar), while only ONC samples appeared in the second cluster (dark grey bar), according to the PCA analysis, where the red and green colors represent high-scale and low-scale intensities, respectively (color-key bar). B Volcano plot of ONC-differentially expressed genes, which represents the number of up- and downregulated genes and the non-significantly changed gene proportions in red, green, and blue, respectively. C A Venn Diagram reveals differential gene activation and overlapping of upregulated genes in three main signaling pathways: PI3K/AKT, Regulation of Actin Cytoskeleton, and Focal Adhesions. The numbers in parentheses indicate the total number of upregulated genes in each one of these pathways. D Dot plot graph of PI3K/AKT pathway upregulated genes. Plot compares 3-day ONC versus 3-week ONC treatments