Skip to main content
Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: Protein kinase B (AKT) upregulation and Thy-1-αvβ3 integrin-induced phosphorylation of Connexin43 by activated AKT in astrogliosis

Fig. 2

PI3K, AKT and Cx43 expression levels in reactive astrocytes. A Astrocytes were treated for 48 h with TNF (10 ng/ml) or left untreated (Control) and messenger RNA (mRNA) was extracted from the samples to measure PI3K, AKT and Cx43 mRNA levels by qRT-PCR. Values indicate fold increase normalized to control values. B Representative Western blots of PI3K, AKT, Cx43 and Hsp90 α/β (loading control) levels in non-treated astrocytes (Control) or astrocytes treated with TNF (TNF). Values indicate the ratio between the densitometric values of the bands and the respective Hsp90 α/β control values. C Histograms of neuronal cell viability (CAD cells) measured by flow cytometry following treatment for 72 h with the conditioned medium obtained from non-treated (Control, red line) or TNF-treated astrocytes (TNF, light blue line). D Quantification of the percentage of neuronal cell viability using eFluor780 in CAD cells, as described in C. Values are mean ± s.e.m. from 3 separate experiments, *p < 0.05 (Mann–Whitney test)

Back to article page