Fig. 3

Enrichment in cell type-specific gene lists for the per-gene lists (a) and WGCNA modules (b). Purple shading represents − log₁₀(P value) where 1.3 corresponds to a P value of 0.05. A higher − log₁₀(P value) corresponds to lower (more significant—darker shades) P value. Non-significant hypergeometric probabilities are displayed as white cells. In a, the results are based on genes differentially expressed in poor 90d mRS IS outcome vs VRFC, good 90d mRS IS outcome vs VRFC, poor 90d mRS IS outcome vs good 90d mRS IS outcome, and genes correlating with 90d NIHSS. In b modules significant for 90-day outcome (mRS poor vs good, and NIHSS) are presented for the ≤ 3 h Network, 5 h Network, and 24 h Network. Blue indicates down-regulated and red up-regulated gene expression with worse outcomes via the beta coefficient for outcome in a linear regression on the module eigengene. Grey indicates modules not significantly associated with the outcome measure. Enrichment of hub gene lists in cell type-specific lists are presented at the bottom. The single asterisk * indicates cell type list from Watkins et al. [21] and the double asterisk ** indicates the cell type list was from Chtanova et al. [20]. Some of the identified Neutrophil genes might be expressed by other granulocytes, i.e., basophils and eosinophils