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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: The FGF/FGFR system in the microglial neuroinflammation with Borrelia burgdorferi: likely intersectionality with other neurological conditions

Fig. 1

Expression of FGFRs in primary rhesus microglia in response to live B. burgdorferi exposure. a Primary microglial cells were exposed to B. burgdorferi for 24 h. Cells were fixed as described in Methods and stained for FGFR1, FGFR2 or FGFR3 by immunofluorescence. The percentage of microglial cells expressing receptors from tissues of 3 different animals were semi-quantitated and graphed. Bar represents standard deviation. ND-not determined. b Immunofluorescent pictures of FGFR1, FGFR2 and FGFR3 staining (green) confirming the expression to be in microglia by additional staining for Iba1 (red). Microglia was derived from a fourth animal tissue. Nuclei are stained blue with DAPI. Increased expression over medium control is also seen. Bar represents 25 µm. c Activation of the FGFR1 pathway is shown by increased expression of phosphoFGFR1 (pFGFR1, green) in microglial cells exposed to B. burgdorferi over medium controls. Bar represents 25 µm. The panel on the far-right shows confocal micrograph of microglia dually stained for Iba1 (red) and pFGFR1 (green) along with the nuclear stain DAPI in blue

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