Fig. 2

Effect of FGFR specific siRNA and FGFR1 inhibitor PD166866 on chemokine and cytokine expression by primary rhesus microglia. The effect of 50 nM siRNA (control siRNA or FGFR specific siRNA) on the secretion of IL-6, CXCL8 and CCL2 is shown in a. Three experiments from microglia derived from tissues of 3 different animals were conducted. Supernatants were analyzed for the indicated inflammatory mediators by multiplex ELISA. A representative graph for siRNA effect on B. burgdorferi induced inflammatory mediator secretion is shown. siRNA effect on medium controls from the same tissue is included. b Shows the effect of FGFR1 inhibitor PD166866 on inflammatory mediator output from primary rhesus microglia in response to B. burgdorferi. A representative experiment is shown for B. burgdorferi along with medium controls from the same animal tissue. Three experiments were carried out on microglia derived from tissues of two different animals. Bar represents standard deviation for both a and b. All statistical comparisons are with Control siRNA or DMSO within each treatment group (B. burgdorferi or Medium). *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001