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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: The FGF/FGFR system in the microglial neuroinflammation with Borrelia burgdorferi: likely intersectionality with other neurological conditions

Fig. 4

Activation of FGFR1 pathway by microglia conditioned medium (a) and likely FGFs present in the conditioned medium in response to B. burgdorferi exposure (b). To test the hypothesis that secreted factors activate the FGFR1 pathway, 24 h supernatants from microglia exposed to either B. burgdorferi or medium alone were collected, filtered, and added to fresh microglial cells from the same tissue for an additional 24 h. Cells were fixed and analyzed for FGFR1 or pFGFR1 by immunofluorescence as before. a Shows the activation of FGFR1 or pFGFR1 (both green) by B. burgdorferi-exposed microglial conditioned media, indicating activation of this pathway through secreted factors. Bar represents 50 µm. As FGFs are the likely ligands for FGFR1 activation, supernatants from microglia derived from 4 different brain tissues were analyzed for FGF secretion by a custom antibody Array (b). The three major types of treatments are labeled below the heatmap. Bb: B. burgdorferi; Med: medium; Bb + PD.: B. burgdorferi + PD166866; additional details as follows: Experiment (Exp.) 1: Bb 10:1 + DMSO, Medium + DMSO, Bb10:1 + 1 µM PD166866. Exp. 2: Bb 10:1 + DMSO, Medium + DMSO, Bb10:1 + 1 µM PD166866. Exp. 3: Bb 10:1, Medium. Exp. 4: Bb 10:1, Medium

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