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Table 1 Mean fold downregulation in inflammatory mediators in response to FGFR inhibition

From: The FGF/FGFR system in the microglial neuroinflammation with Borrelia burgdorferi: likely intersectionality with other neurological conditions

Inhibitor/treatment

IL-6

CXCL8

CCL2

Bb 10:1/siRNA

FGFR1 siRNA (50 nM)

1.725 (± 0.565)

1.151 (± 0.082)

1.212 (± 0.111)

FGFR2 siRNA (50 nM)

2.224 (± 0.916)

1.214 (± 0.068)

1.324 (± 0.151)

FGFR3 siRNA (50 nM)

1.535 (± 0.325)

1.274 (± 0.125)

1.437 (± 0.321)

Medium/siRNA

FGFR1 siRNA (50 nM)

0.973 (± 0.112)

1.073 (± 0.044)

1.439 (± 0.299)

FGFR2 siRNA (50 nM)

1.188 (± 0.070)

1.194 (± 0.189)

1.534 (± 0.258)

FGFR3 siRNA (50 nM)

1.278 (± 0.165)

1.571 (± 0.381)

1.345 (± 0.123)

Bb 10:1/FGFR1 inhibitor

PD166866

5 µM

1.982 (± 0.394)

1.954 (± 0.492)

3.292 (± 1.056)

2.5 µM

1.850 (± 0.737)

1.906

(± 0.683)

2.769 (± 1.397)

1 µM

1.703 (± 0.474)

1.713 (± 0.543)

2.077 (± 0.759)

500 nM

1.071 (± 0.367)

0.901 (± 0.226)

1.113 (± 0.352)

Medium/FGFR1 inhibitor

PD166866

5 µM

0.880 (± 0.062)

1.099 (± 0.110)

2.322 (± 0.615)

2.5 µM

1.133 (± 0.133)

1.213 (± 0.172)

2.201 (± 0.417)

1 µM

1.303 (± 0.048)

1.217 (± 0.114)

1.669 (± 0.125)

500 nM

1.138 (± 0.129)

1.012 (± 0.092)

1.402 (± 0.119)

Bb 10:1/BGJ398

1 µM

2.292 (± 0.140)

2.046 (± 0.158)

3.480 (± 0.568)

500 nM

2.403 (± 0.515)

1.409 (± 0.233)

1.836 (± 0.301)

Bb 10:1/AZD4547

10 µM

3.817 (± 1.882)

2.369 (± 1.173)

6.034 (± 3.967)

5 µM

2.176 (± 0.921)

2.048 (± 0.942)

2.809 (± 1.495)

Medium/AZD4547

10 µM

1.424 (± 0.937)

1.929 (± 0.101)

5.656 (± 1.626)

5 µM

2.170 (± 0.581)

1.338 (± 0.214)

2.432 (± 0.434)

  1. Fold change was calculated as treatment + (control siRNA or DMSO)/treatment + (FGFR siRNA or inhibitor) for each experiment. Average fold change across experiments with standard error of the mean in shown in brackets. Numbers greater than 1 indicate a downregulation of inflammatory mediator, while numbers less than once indicate an increase. Numbers that are bold indicate a statistically significant downregulation of chemokines/cytokines in most of the experiments. Other numbers indicate no statistically significant or conclusive effects when all experiments are considered
  2. SiRNA: average fold change calculated from 3 experiments in microglia derived from 3 cortex tissues
  3. FGFR1 inhibitor PD166866: average fold change calculated from 3 experiments in microglia derived from 2 cortex tissues for B. burgdorferi and 2–3 experiments in microglia derived from 2 cortex tissues for medium controls
  4. BGJ398: average fold change calculated from 3 to 4 experiments in microglia derived from 3 cortex tissues. For medium samples some of the fold changes could not be calculated due to undetectable values. The experiments are shown in Additional file 1: SM3a
  5. AZD4547: average fold change calculated from 3 experiments in microglia derived from 3 cortex tissues for B. burgdorferi and 2–3 experiments in microglia derived from 3 cortex tissues for medium controls