Fig. 2

LND inhibits inflammasome activation in vivo. A and B Immunoblot (A) and quantification of gray values (B) of GSDMD, cleaved caspase-1 and cleaved IL-1β in brain tissue at 23 h of reperfusion after MCAO n = 3. C and D Immunohistochemical staining (C) and quantitative analysis (D) of GSDMD and caspase-1 at 23 h of reperfusion after MCAO in the cortex and striatum of the ischemic area. n = 5. Scale bar = 100 μm. E and F Immunohistochemical staining (E) and quantitative analysis (F) of GSDMD and caspase-1 in spinal cord tissue after EAE induction. n = 7. Scale bar = 100 μm. G and H ELISA analysis of IL-1β (G) and IL-18 (H) in the serum of mice that were intraperitoneally injected with LPS (15 mg/kg) for 4 h with or without pretreatment with LND. n = 15. The data are expressed as the mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001; NS, not significant. The data were analyzed by one-way ANOVA and Tukey’s test