Fig. 6
From: Directly targeting ASC by lonidamine alleviates inflammasome-driven diseases
LND directly binds to ASC to inhibit NLRP3 assembly. A The docking model of ASC with LND using ligand scout software. LND is shown as a stick and colored green. The CARD domain of ASC is shown in cartoon and colored pink, while the interaction sites are colored orange. Yellow lines represent hydrogen bonds. B Sensorgrams showing LND binds to recombinant ASC protein were obtained from SPR analysis using Biacore. Different concentrations of LND are presented as an overlay plot aligned at the start of the injection. C LPS-primed BMDMs were lysed and incubated with LND overnight at 4 °C. DARTS assays were performed with pronase (20 ng/μg protein), and lysates were analyzed by immunoblotting