Fig. 1

Expression and distribution of PGRN in the NAc following NAc neuroinflammation. A, B Representative immunofluorescence images showing the exact site and diffusion degree after NAc nuclei injection (scale bar 500 μm). C RT-PCR analysis showed PGRN mRNA was increased 4 h after NAc LPS injection, and its expression was continuously and drastically enhanced 24 h after NAc LPS administration compared to that after control treatment. D, E A large of PGRN was located at NeuN-labeled neurons in the NAc under normal circumstance, but decreased PGRN expression in the neurons from NAc was observed during NAc LPS challenge. F, G Compared to sporadic scattered of PGRN was expressed in the GFAP-labeled astrocytes in the NAc from NAc saline-treated mice, NAc LPS exposure obviously increased PGRN expression in astrocyte of NAc in mice. H, I Likewise, distribution of PGRN in the Iba1-labeled microglia from the NAc was significantly increased after NAc LPS exposure, when compared with the scarcely any PGRN expression in the microglia of NAc from NAc saline-treated mice. J–L The statistical numbers of co-localized between NeuN-labeled neurons, GFAP-labeled astrocyte or Ibai-labeled microglia and PGRN-positive cells in the NAc in NAc saline-treated and NAc LPS-treated mice. NeuN, marker of neurons; GFAP, marker of astrocytes; Iba1, marker of microglia; nuclei, stained with DAPI (blue); scale bar: 100 µm (D–I). n = 6 per group. Data were analyzed by Student’s t-test, and expressed as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001