Fig. 6

Effects of p38 and NF-κB signaling pathways on PGRN deficiency during NAc neuroinflammation. A–D Western blot analysis showed that ERK MAPK signaling pathway had no effects in the WT and PGRNKO mice after NAc LPS challenge. E–H Western blot analysis showed that JNK MAPK signaling pathway did not alter between WT and PGRNKO mice after NAc LPS injection. I–L The expression of p38 and p-p38 were increased and activation of p38 (p-p38/p38) was decreased in the NAc after NAc LPS injection as compared to that in NAc saline-treated mice, but these changes in p38 and p-p38 expression without p38 activation in the NAc were abolished in NAc LPS-treated PGRNKO mice. M–P The expression in p-p65 and p65 but no p65 activation (p-p65/p65) in the NAc was obviously elevated in the NAc LPS-treated WT mice relative to control mice, which was reversed in the NAc LPS-treated PGRNKO mice. n = 6 per group. Data were analyzed by one-way ANOVA followed by the LSD multiple comparisons test, and expressed as mean ± SEM. *p < 0.05, **p < 0.01