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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Expansion of regulatory T cells by CD28 superagonistic antibodies attenuates neurodegeneration in A53T-α-synuclein Parkinson’s disease mice

Fig. 3

Assessment of target engagement by expansion of Treg after CD28SA injection. A Representative FACS analysis images showing the CD4-gated CD25+FoxP3+ Treg populations in cervical lymph nodes (CLN), spleen and brain of empty vector (EV) or hαSyn vector-injected mice treated either with PBS or with CD28SA, 3 days after respective i.p. injections. B-D Treg percentages in CLN (B), spleen (C) and brain (D). E–G IL-10 concentrations in CLN (E), spleen (F) and brain (G). Statistical analysis by one-way ANOVA followed by Tukey’s multiple comparisons test, B-D CLN, F(3, 20) = 255.7, P < 0.0001; spleen, F(3, 20) = 46.26, P < 0.0001; brain, F(3, 20) = 20.22, P < 0.0001.; G brain, F(3, 20) = 0.643, P = 0.5963. E, F Kruskal–Wallis test followed by Dunn’s multiple comparisons: CLN, H = 17.93, P = 0.0005; spleen: H = 19.47, P = 0.0002. *P < 0.05, **P < 0.01, ***P < 0.001. All data are shown as mean ± SEM. n = number of biologically independent samples, each sample consisting of pools of two animals

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