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Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: Expansion of regulatory T cells by CD28 superagonistic antibodies attenuates neurodegeneration in A53T-α-synuclein Parkinson’s disease mice

Fig. 6

CD28SA decreases activation state of brain T cells in hαSyn PD mice. A, B Representative FACS analysis images showing the proportion of CD4+CD69+ (A) and CD8+CD69+ (B) activated T cells in the brain of EV- or hαSyn-injected mice treated either with PBS or with CD28SA. C, D Percentages of CD4+CD69+ (C) and CD8+CD69+ (D) in the brain of the indicated groups as analyzed by FACS. E-J Cytokine concentrations in the brain: IL-2 (E), IL-4 (F), IL-5 (G), IL-13 (H), IL-17A (I) and IFN-γ (J). Statistical analysis by one-way ANOVA followed by Tukey’s multiple comparisons test: CD4+CD69+, F(3, 20) = 16.82, P < 0.0001; CD8+CD69+, F(3, 20) = 22.16, P < 0.0001. Statistical analysis by Kruskal–Wallis test followed by Dunn’s multiple comparisons test: IL-2, H = 13.16, P = 0.0043. *P < 0.05, **P < 0.01, ***P < 0.001. All data are shown as mean ± SEM. n = number of biologically independent samples, each sample consisting of pools of two animals

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