Fig. 6From: Teriflunomide as a therapeutic means for myelin repairTeriflunomide mediated mitochondrial changes in oligodendroglial cells within the corpus callosum. A, B″ Representative images of Sox10 and Tom20 double positive cells in the caudal corpus callosum after 1 week of remyelination. A, B″, C Extent of mitochondrial changes was revealed as the percentage of Tom20-expressing area in the defined region of interest (white borders). Further morphometric quantifications were assessed to determine mitochondrial, D length, E number and F mean form factor (FF). FF = 1 indicates round object, hence low length of mitochondrial networks within cells (form factor 1; low) and increases with elongation, to multiple mitochondria exhibiting elongated tubules (form factor > 1 complex). G–G″ Moreover, the relative percentage of double positive cells (Sox10+ /Tom20+) containing few and less-elongated Tom20+ mitochondria categorized as “low” (blow up; asterix in B) where compared to Sox10-positive cells exhibiting Tom20+ mitochondria with elongated mitochondrial networks categorized as “complex” (blow ups; asterix in B′, B″. G′ Individual data points related to the determination of double positive cells (Sox10 +/Tom20 +) categorized as “low” and (G″) individual data points for double positive cells (Sox10 +/Tom20 +) categorized as “complex”. Number of animals per analysis n = 6. Data are shown as mean values (horizontal lines), while error bars represent standard error of the mean (SEM; vertical lines). Plots also show all individual data points. Significance was assessed using Tukey’s range test following one-way ANOVA (C–G). Data were considered statistically significant (95% confidence interval) at *p < 0.05, **p < 0.01, ***p < 0.001. Scale bars: 50 µm, 20 µm, 10 µmBack to article page