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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Intracellular deposits of amyloid-beta influence the ability of human iPSC-derived astrocytes to support neuronal function

Fig. 3

Co-cultures of neurons and Aβ-exposed astrocytes contain fewer apoptotic cells. A Study design to investigate the effects of cell-to-cell interaction between control or Aβ astrocytes and neurons (created with www.Biorender.com). B, C Representative images of control and Aβ co-cultures, respectively (scale bar: 20 μm). D Images generated by the apoptotic cell analysis pipeline in CellProfiler™. Live cells (blue) and dead cells (green) were overlayed to provide the dead/live percentage. E Astrocytes (white) surrounded the clumps of neuronal cell corpses (green) and live neurons (orange) (scale bar: 50 μm). F The percentage of apoptotic cells was decreased in Aβ co-cultures compared to control co-cultures and neuronal monocultures. G Astrocytic area% as measured by vimentin + staining displayed no difference between control and Aβ co-cultures. H Neuronal area% as measured by MAP2 + staining was increased in Aβ co-cultures, compared to control co-cultures and neuronal monocultures (Results in all graphs are presented as mean ± S.E.M. from three independent experiments; ns = non-significant, *p < 0.05, ****p < 0.0001)

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