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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: Intracellular deposits of amyloid-beta influence the ability of human iPSC-derived astrocytes to support neuronal function

Fig. 4

Conditioned media, but not EVs affect overall viability in neuronal monocultures. A Study design to investigate the effect of conditioned media and EVs from control and Aβ-exposed astrocytes on neuronal monocultures (created with www.Biorender.com). B EVs were isolated from astrocytic media via a series of centrifugation and ultracentrifugation steps. (B1) TEM analysis revealed characteristic EV morphology (scale bar: 200 nm). C Overall viability was increased in neuronal monocultures that received ACM from control astrocytes, compared to control cultures and cultures that received Aβ ACM. D No difference in viability was observed in neuronal monocultures treated with EVs from control or Aβ-exposed astrocytes [Results are presented as mean ± S.E.M. from three (C) and two (D) independent experiments, respectively; values are normalized to the control neuronal monocultures and control EV monocultures for C and D, respectively; ns = non-significant, **p < 0.01, ****p < 0.0001]

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Journal of Neuroinflammation

ISSN: 1742-2094