Fig. 8

Conditioned media from astrocytes with Aβ inclusions increase neuronal synaptic activity. A The frequency of sEPSCs was significantly increased in neuronal monocultures treated with Aβ ACM compared to monocultures treated with control ACM. B The amplitude of sEPSCs was also increased in Aβ ACM cultures. D, E No difference was observed in the frequency and amplitude of mEPSCs in monocultures exposed to Aβ ACM compared to cultures exposed to control ACM. C, F Representative traces of gap-free recordings. Red arrowheads indicate the position of sEPSCs and mEPSCs, respectively. (Results are presented as mean ± S.E.M.; neurons + ACM: n = 17 cells out of two batches, neurons + Aβ ACM: n = 16 cells out of two batches; *p < 0.05)