Fig. 4

NK cell migration is CXCR4-dependent. A Testing the Ncr^iCre efficiency in the NKp46⁺ cells by FACS using the Rosa^tdTomato reporter mice (n = 3). B Analysis of the NKp46⁺ cell populations in the Ncr^iCre; Rosa^tdTomato reporter mice (n = 3). Representative dot plots at P15 are shown. C Cxcr4 mRNA levels in sorted NK cells from Control (Cxcr4^fl/fl) and KO (Cxcr4^Ncr1−/−) mice. RNA in each group was extracted from NK cells sorted from pooled lymph node and spleen suspensions from 3 mice. D Flow cytometry analysis of ILCs in the stroke hemisphere from Control (Cxcr4^fl/fl) and KO (Cxcr4^Ncr1−/−) mice at P15, NK (CD45⁺Lin⁻NKp46⁺NK1.1⁺CD49a⁻CD49b⁺), intILC1 (CD45⁺Lin⁻NKp46⁺NK1.1⁺CD49a⁺CD49b⁺), ILC1 (CD45⁺Lin⁻NKp46⁺NK1.1⁺CD49a⁺CD49b⁻), ILC2 (CD45⁺Lin⁻NKp46⁻NK1.1⁻CD127⁺ST2⁺KLRG1⁺) and NKp46⁺ ILC3 (CD45⁺Lin⁻NK1.1⁻NKp46⁺CD127⁺). Representative dot plots of ILCs at P15 (n = 3 mice) are shown. E Quantification of ILCs in the stroke hemisphere from WT (Cxcr4^fl/fl) and KO (Cxcr4^Ncr1−/−) mice at P0, P2 and P15. Data represent n = 3 mice. NK P2 **P = 0.0085, P15 ****P < 0.0001. F Analysis at P0 (n = 3 mice), 2 (n = 4 mice) of the CXCR4⁺ NK cells at the top and total NK cells (Lin⁻CD45⁺NKp46⁺NK1.1⁺CD49b⁺) at the bottom in blood of PT-treated mice showed a significant increase of CXCR4 ⁺ NK cells at P2 and P15 within the blood, while total NK cells were significantly increased only at P15