Fig. 2

SS-31 attenuates pyroptosis after SCI. A Double-IF staining of Caspase-1 and NeuN in the spinal cords of the Sham, SCI, and SCI + SS-31 groups (scale bar = 25 μm). B Quantified Caspase-1 IF data are presented on the right. C Double-IF staining of NLRP3 and NeuN in the spinal cords of the indicated groups (scale bar = 25 μm). D Quantified NLRP3 IF data are presented on the right. E WB for Caspase-1, GSDMD-N, NLRP3, NLRP1, ASC, IL-1β, IL-18 and their expression levels in the Sham, SCI, and SCI + SS-31 groups. GAPDH was utilized as a loading control. The gels were run under the same experimental conditions, and the cropped blots are shown here. F The optical density values of Caspase-1, GSDMD-N, NLRP3, NLRP1, ASC, IL-1β and IL-18 were quantified and analysed in each group. The data are shown as the mean ± SEM. n = 5. *P < 0.05, **P < 0.01. ns indicates no significance