Table 2 List of the antibodies used for flow cytometry (FC) and immunohistochemistry (IHC) analyses
From: Blocking interleukin-23 ameliorates neuromuscular and thymic defects in myasthenia gravis
Antibody target | Conjugate | Host | Reactivity | Clone | Supplier | Used in |
|---|---|---|---|---|---|---|
CCR4 | PE | Mouse | Human | 205410 | R&D | FC |
CCR6 | APC | Mouse | Human | 11A9 | BD Bioscience | FC |
CD127 | FITC | Mouse | Human | ebioRDR5 | eBioscience | FC |
CD19 | FITC | Mouse | Human | HIB19 | eBioscience | FC |
CD25 | PE | Mouse | Human | BC96 | eBioscience | FC |
CD4 | V450 | Mouse | Human | RPA-T4 | BD Bioscience | FC |
CD45 | V500 | Mouse | Human | H130 | BD Bioscience | FC |
CD8 | ACP-H7 | Mouse | Human | SK1 | BD Bioscience | FC |
IL-23R | PerCP | Mouse | Human | 218213 | R&D Systems | FC |
Keratin 5 | Purified | Rabbit | Human | Poly19055 | BioLegend | IHC |
Keratin 14 | Purified | Rabbit | Human | Poly19053 | BioLegend | IHC |
CCR6 | APC | Rat | Mouse | 140706 | BD Pharmingen | FC |
CD127 | V450 | Rat | Mouse | SB199 | BD Bioscience | FC |
CD25 | FITC | Rat | Mouse | 16184 | BD Bioscience | FC |
CD31 | Purified | Rat | Mouse | MEC 13.3 | BD Pharmingen | IHC |
CD4 | APC-eF780 | Rat | Mouse | GK 1.5 | eBioscience | FC |
CD62L | PerCP Cy5.5 | Rat | Mouse | MEL14 | eBioscience | FC |
CD8 | PE Cy7 | Rat | Mouse | 53–6.7 | BD Bioscience | FC |
Anti-rabbit IgG | Alexa Fluor 594 | Chicken | Rabbit | N/A | Invitrogen | IHC |
Anti-rat IgG | Alexa Fluor 488 | Chicken | Rat | N/A | Invitrogen | IHC |