Fig. 6

Increased inflammation and reduced tight junctions of the colon in CUMS mice. a Immunofluorescence assay for Claudin-1 (red) and DAPI (blue) expression in the colon of mice (× 40 magnifications). Morphological changes in the colon, as seen with H&E and AB-PAS staining (× 40 magnifications). Scale bar: 200 μm. b–f Western blotting analysis and quantification data of MUC2, Claudin-1, Occludin and ZO-1 in the colon of mice. g–j mRNA expression of MUC2, Claudin-1, Occludin and ZO-1 in the colon of mice. Each value represents the mean ± SEM. *p < 0.05, **p < 0.01 and ***p < 0.001 means difference of WT Control vs. WT CUMS, TPH2 KO vs. TPH2 KO CUMS, TPH2 KO vs. WT CUMS + FLX at the same point