Fig. 2

iVEC transplantation decreased CD4⁺ T cells in ischemic white matter infarct. Immunohistochemistry with a CD4 antibody was performed to quantify the number of CD4⁺ T cells. Nuclei were stained by Hoechst 33342. A A typical bright field image of the white matter infarct on day 7 (top). Scale bar: 500 µm. Immunohistochemical images for CD4 under higher magnification of the ipsilateral ischemic region (left) and the contralateral intact region (right). Scale bar: 50 µm. B Representative immunohistochemical images for CD4 in iVEC-transplanted and BSA-injected brains at days 10, 14, and 21. Scale bar: 50 µm. C Quantification of the CD4⁺ cell number in iVEC-transplanted and BSA-injected brains at days 10, 14, and 21. All data are expressed as mean ± SEM. n = 3–5 in each group. ^*p < 0.05. ns, no significant difference. ^###p < 0.001 compared to Day 7 group. These experiments were repeated three times, and similar results were obtained each time. Typical experiments are shown here