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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Transplanted human iPSC-derived vascular endothelial cells promote functional recovery by recruitment of regulatory T cells to ischemic white matter in the brain

Fig. 3

iVEC transplantation increased the Foxp3+ Tregs in ischemic white matter infarct. Immunohistochemistry with a Foxp3 antibody was performed to quantify the number of Foxp3+ Tregs. Nuclei were stained by Hoechst 33342. A A typical bright field image of the white matter infarct on day 7 (top). Scale bar: 500 µm. Immunohistochemical images for Foxp3 under higher magnification of the ipsilateral ischemic region (left) and the contralateral intact region (right). Scale bar: 50 µm. B Representative immunohistochemical images for Foxp3 in iVEC-transplanted and BSA-injected brains at days 10, 14, and 21. Scale bar: 50 µm. C Quantification of the Foxp3+ cell number in iVEC-transplanted and BSA-injected brains at days 10, 14, and 21. All data are expressed as mean ± SEM. n = 3–5 in each group. *p < 0.05 and **p < 0.01. #p < 0.05 and ###p < 0.001 compared to Day 7 group. These experiments were repeated three times, and similar results were obtained each time. Typical experiments are shown here

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