Fig. 5

FTY720 treatment suppressed inflammatory response but did not promote recovery of the white matter infarct. FTY720 administration from days 7 to 14 after stroke onset was performed to inhibit the egress of T lymphocytes from lymph nodes. A Representative immunohistochemical images for ED-1 in vehicle-injected (Vehicle), iVEC-transplanted (iVEC), FTY720-treated (FTY), and FTY720-treated and iVEC-transplanted (FTY + iVEC) groups at day 14. Nuclei were stained by Hoechst 33342. Scale bar: 200 µm. B Quantification of fluorescence intensity of ED-1 staining in each group at day 14. All data are expressed as mean ± SEM. n = 5 in each group. ^**p < 0.01 and ^***p < 0.001. C Representative LFB staining images of rat brain sections in each group. Scale bar: 500 µm. D Quantification of the damaged IC volume in each group on day 14. All data are expressed as mean ± SEM. n = 5 in each group. ^*p < 0.05 and ^***p < 0.001. These experiments were repeated three times, and similar results were obtained each time. Typical experiments are shown here