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Table 1 Antibodies used for immunohistochemistry. Anti-mouse CD3, Rabbit/IgG, clone SP162, dilution 1:150, company Abcam, Cambridge, UK

From: The presence of cerebellar B cell aggregates is associated with a specific chemokine profile in the cerebrospinal fluid in a mouse model of multiple sclerosis

Antibody

Species/isotype

Conjugate

Clone

Dilution

Company

Anti-mouse CCR5

Rabbit/ IgG

 

Polyclonal

1:125

Novus Biologicals, Littleton, CO, USA

Anti-mouse CD3

Rat/ IgG1

 

CD3-12

1:100

Abcam, Cambridge, UK

Anti-mouse CD45R (B220)

Rat / IgG2a, κ

 

RA3-6B2

1:250

Thermo Fisher Scientific, Waltham, MA, USA

Anti-mouse CXCR5

Rabbit

 

Polyclonal

1:500

Merck, Darmstadt, Deutschland

Anti-mouse CXCR6

Rabbit/ IgG

 

Polyclonal

1:150

Novus Biologicals, Littleton, CO, USA

Anti-mouse F4/80

Rat/ IgG2b

 

CI:A3-1

1:100

Abcam, Cambridge, UK

Anti-rabbit IgG (H + L)

Goat, IgG

Biotin

Polyclonal

1:500

Abcam, Cambridge, UK

Anti-rat IgG (H + L)

Goat

Biotin

Polyclonal

1:500

Abcam, Cambridge, UK

Anti-rabbit IgG (H + L)

Donkey

Cyanine (Cy)â„¢3

Polyclonal

1:300

Jackson Immuno Research Labs, West Grove, PA, USA