Fig. 3
MgH2 treatment alleviates inflammation in the hippocampus and corpus callosum (CC) of EAE mice. A–C Representative anti-Iba1 (red) and anti-GFAP (purple) immunofluorescence of DG, CA1, and CA3 zones in the hippocampus. White arrows mark Iba1+ microglia and GFAP+ astrocytes. D Quantitative analysis of Iba1+ cells/mm2 of DG (F = 0.2353), CA1 (F = 0.1947) and CA3 (F = 0.1449) zone in the hippocampus, n = 3 mice per group. E Quantitative analysis of GFAP+ cells/mm2 of DG (F = 0.6708), CA1 (F = 0) and CA3 (F = 0.07) zone in the hippocampus, n = 3 mice per group. F Representative anti-Iba1 (red), anti-GFAP (purple), and anti-Olig2 (green) immunofluorescence of the CC, LV: lateral ventricle. G–I Quantitative analysis of Iba1+ cells/mm2 (F = 0.6852), GFAP+ cells/mm2 (F = 3.2429), and Olig2+ cells/mm2 (F = 0.81) of the CC, n = 3 mice per group. J Representative anti-MBP (red), anti-NF200 (green), and anti-SMI32 (purple) immunofluorescence of the CC. The difference of SMI32+ area is shown in white boxes. K–M Quantitative analysis of median fluorescence intensity (MFI) of MBP (F = 14.0746), NF200 (F = 4.5282), and SMI32 (F = 15.8079), n = 3 mice per group. White arrows mark Iba1+ or GFAP+ cells. Data are presented as mean ± SEM