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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: Nanoparticulate MgH2 ameliorates anxiety/depression-like behaviors in a mouse model of multiple sclerosis by regulating microglial polarization and oxidative stress

Fig. 5

MgH2 treatment reduces inflammation of the hippocampus and corpus callosum after 24-h restraint. A–C Representative anti-Iba1 (red), anti-GFAP (purple) immunofluorescence of DG, CA1, and CA3 zones in the hippocampus. White arrows mark Iba1+ microglia and GFAP+ astrocytes. D Quantitative analysis of Iba1+ cells/mm2 of DG (F = 81.5234), CA1 (F = 43.3062) and CA3 (F = 64.6975) zones in the hippocampus, n = 3 mice per group. E Quantitative analysis of GFAP+ cells/mm2 of DG (F = 77.5573), CA1 (F = 37.1143) and CA3 (F = 25.7619) zones in the hippocampus, n = 3 mice per group. F Representative anti-Iba1 (red), anti-GFAP (purple), and anti-Olig2 (green) immunofluorescence of the CC. White arrows mark Iba1+ microglia and GFAP+ astrocytes. G–I Quantitative analysis of Iba1+ cells/mm2 (F = 14.4151), GFAP+ cells/mm2 (F = 0.9667) and Olig2+ cells/mm2 (F = 0.7709) of the CC, n = 3 vs. 3 mice per group. White arrows mark Iba1+ or GFAP+ cells. Data are presented as mean ± SEM

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