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Fig. 7 | Journal of Neuroinflammation

Fig. 7

From: Nanoparticulate MgH2 ameliorates anxiety/depression-like behaviors in a mouse model of multiple sclerosis by regulating microglial polarization and oxidative stress

Fig. 7

MgH2 treatment can affect polarization and inhibit oxidative stress of microglia. A Schematic diagram displaying the study on the polarization of microglia in vitro. B Relative inos (F = 4.3253), Tnf-α (F = 4.7244), Arg-1 (F = 15.4525), Ym1 (F = 6.8116), and Fizz1 (F = 9.5084) mRNA expression in microglia in vitro at 24 h after IL-4 or LPS stimulation. inos and Tnf-α are used as M1 markers; Arg-1, Ym1, and Fizz1 are used as M2 markers. n = 3 experiments per group. C Western blotting analysis and relative iNOS levels of microglia in vitro at 72 h (F = 5.3167), n = 3 experiments per group. D Western blotting analysis and relative ARG-1 protein levels of microglia in vitro at 72 h (F = 6.0097), n = 3 experiments per group. E, F Intracellular ROS of microglia is measured by flow cytometry (DCFH-DA fluorescent probe) and quantitative analysis of the MFI (F = 10.4918). n = 3 experiments per group. G, H Representative graphs show JC-1 fluorescence of microglia in vitro and quantitative analysis of green/red fluorescence intensity (%), red fluorescence represents the mitochondrial aggregate JC-1 and green fluorescence indicates the monomeric JC-1 (F = 0.2891). n = 3 experiments per group. Data are presented as mean ± SEM

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