Fig. 4
The increase in KCC2 expression and activity is found in dorsal CA1 of adult mice neonatally treated with LPS. CON and LPS represent control mice and mice neonatally treated with LPS (LPS mice), respectively. A Timeline of the experimental approach. B–E Top, representative immunoblots for GABAARα1 (B), GABAARα2 (C), NKCC1 (D) and KCC2 (E) in protein extracts from samples of control and LPS mice. β-actin was used as an internal standard. Bottom, quantification of GABAARα1 (B), GABAARα2 (C), NKCC1 (D) and KCC2 (E) in samples from control and LPS mice. F Left, representative image of immunohistostaining of KCC2. KCC2 is shown in green (white arrows). Right, the relative intensity of KCC2 in dorsal CA1 of control and LPS mice. Scale bar = 25 μm. G Example current–voltage curves of IPSC recorded at different holding potentials from − 120 to − 20 mV in pyramidal neurons from control and LPS mice. Insets show sample traces of IGABA recorded in control (green) and LPS (blue) groups. EGABA is shown at the arrow. H and I EGABA (H) and driving force (I) measured in control and LPS groups. In B–F, H and I, circles indicate single data points, and their averages (± s.e.m.) are shown as columns in B–F or bars in H and I. Statistical analysis: Student’s t test. *P < 0.05, **P < 0.01 and ***P < 0.001