Fig. 5

KCC2 overexpression is associated with TGF-β1 decrease in the dorsal CA1 region of adult mice neonatally treated with LPS. CON and LPS represent control mice and mice neonatally treated with LPS (LPS mice), respectively. A Timeline of the experimental approach. B and D Quantification of mRNA expressions of inflammatory factors in samples from P8 (B) or P80 (D) control and LPS mice. C Top, representative immunoblots for KCC2 in protein extracts from samples of P8 control and LPS mice. β-actin was used as an internal standard. Bottom, quantification of KCC2 in samples from P8 control and LPS mice. E Top, representative immunoblots for TGF-β1 in protein extracts from samples of P80 control and LPS mice. β-actin was used as an internal standard. Bottom, quantification of TGF-β1 in samples from P80 control and LPS mice. F Pearson analysis of the correlation between KCC2 and TGF-β1 protein in samples from P80 control and LPS mice. In B–F, circles indicate single data points. In B–E, columns represent the averages (± s.e.m.) of the data. Statistical analysis: Student’s t test in B–E, and Pearson analysis in F. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001