Fig. 6
TGF-β1 vector treatment rescues KCC2 expression and function in the dorsal CA1 of adult mice neonatally treated with LPS. CON and LPS represent control mice and mice neonatally treated with LPS (LPS mice), respectively. A Timeline of the experimental approach. B Left, abundant expression of eGFP (green) in the dorsal CA1 region. Scale bar = 500 μm. Right, expression of eGFP in cells in the CA1 region with the white dashed box in the left panel. Scale bar = 50 μm. C–G: Top, representative immunoblots for TGF-β1 (C), GABAARα1 (D), GABAARα2 (E), NKCC1 (F) and KCC2 (G) in protein extracts from samples of control and LPS mice treated with control vector or TGF-β1 vector. β-actin was used as an internal standard. Bottom, quantification of TGF-β1 (C), GABAARα1 (D), GABAARα2 (E), NKCC1 (F) and KCC2 (G) in samples from control and LPS mice treated with control vector or TGF-β1 vector. H and I EGABA (H) and driving force (I) measured in each experimental group. In C–I, circles indicate single data points, and their averages (± s.e.m.) are shown as columns in C–G or bars in H and I. Statistical analysis: two-way ANOVA followed by Bonferroni. *P < 0.05, **P < 0.01 and ***P < 0.001