Fig. 8

Hypermethylation at TGFb1 promoter is responsible for the decreased TGF-β1 expression in adult mice neonatally treated with LPS. CON and LPS represent control mice and mice neonatally treated with LPS (LPS mice), respectively. A Timeline of the experimental approach for obtaining the results in B–D. B and C Quantification of 5-mC (B) and 5-hmC (C) at TGFb1 promoter from samples of control and LPS mice. D Pearson analysis of the correlation between 5-mC at TGFb1 promoter and the corresponding transcripts in samples from adult control and LPS mice. E Timeline of the experimental approach for obtaining the results in F–H. F Quantification of 5-mC at TGFb1 promoter from samples of adult control and LPS mice treated with VEH or 5-aza-CdR (AZA). G: Quantification of TGF-β1 mRNA expression from samples of adult control and LPS mice treated with VEH or AZA. H Top, representative immunoblots for TGF-β1 in protein extracts from samples of control and LPS mice treated with VEH or AZA. β-actin was used as an internal standard. Bottom, quantification of TGF-β1 protein in samples from control and LPS mice treated with VEH or AZA. In B–D and F–H, circles indicate single data points. In B, C and F–H, columns represent the averages (± s.e.m.) of the data. Statistical analysis: Student’s t test in B and C, Pearson analysis in D, and two-way ANOVA followed by Bonferroni in F–H. *P < 0.05, and ***P < 0.001