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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Regulatory T cells decrease C3-positive reactive astrocytes in Alzheimer-like pathology

Fig. 3

Modulation of Tregs does not impact the morphology and branching complexity of plaque-associated astrocytes. A Illustration of the 3D modeling process for analyzing astrocytes’ branching complexity around amyloid deposits. Top panel: 3D modeling of astrocytes branching hierarchy. Bottom panel: representative image of modeled astrocytes and amyloid plaques. Astrocytes in the vicinity of amyloid deposits are colored according to the size of their corresponding amyloid plaque, unrecruited astrocytes are represented in cyan. B Quantification of astrocytes’ bounding box volume in close vicinity to amyloid deposits of different size ranges in the cerebral cortex of APPPS1 mice treated for either the depletion (top) or amplification of Tregs (bottom). C Sholl analysis of the number of intersections between astrocyte branches and consecutive concentric spheres centered on astrocytes’ soma and of a radius period of 1 µm, of APPPS1 mice treated for either the depletion of Tregs (top) or the amplification of Tregs (bottom). Results are represented according to the size of amyloid deposits astrocytes are recruited towards. Unrecruited astrocytes are referenced as Ø. Mean +/− SEM (n > 30 amyloid deposits from 4–6 mice/ group). Mann–Whitney test (A-B): *P < 0.05; **P < 0.01; ***P < 0.001. Linear regression (C): *P < 0.05; **P < 0.01; ***P < 0.001

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