Fig. 4

Siponimod affects the sequential expression of cytokines and transcription factors mRNAs expression in the popliteal lymph nodes and the cauda equina. The bar graphs show the expression of IFN-γ, IL-10, IL-17, and Foxp3 mRNAs in the popliteal lymph nodes (LN) and in the cauda equine (CE) at days 9, 12, 15, 21, and 28 p.i.. Data for A IFN-γ, C IL-10, E IL-17, and G Foxp3 were data of LN. B IFN-γ, D IL-10, F IL-17, and H Foxp3 mRNAs in CE. Data show mean ± SEM. *p < 0.05: EAN vs. siponimod groups. Statistics analysis using Mann–Whitney U-test