Fig. 5

TMEM119⁺/MHC-II⁺ EVs are significant increased 28 days following ET-1 injection. A MHC-II IHC in selected regions of ET-1 and saline injected animals: injection site, adjacent corpus callosum (CC) and contralateral striatum. Scale bar indicates 100 µm. B Images taken at × 40 magnification depicting microglia morphology within stroke region, scale bar indicates 25 μm C TMEM119⁺MHC-II⁺ labelled events/μL of plasma from baseline and 28-day samples of saline injection group. D TMEM119⁺MHC-II⁺ labelled events/μL of plasma from baseline and 28-day samples of ET-1 injection group. E TMEM119⁺/MHC-II⁺ labelled events/µL of plasma from 28-day samples of ET-1 and saline injection groups. # Indicates statistical significance (p < 0.05) measured using Wilcoxon paired rank test. *Indicates statistical significance (p < 0.05) measured using Mann–Whitney test