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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: ASC specks exacerbate α‑synuclein pathology via amplifying NLRP3 inflammasome activities

Fig. 2

ASC fibrils amplified NLRP3 inflammasome activation in PFFs-treated microglia. a IB was probed for purified ASC proteins. 22 KDa: theoretical molecular weight of ASC monomer. b Representative transmission electron microscopy (TEM) images of ASC fibrils induced by incubation. c Schematic presentation of the following experimental setup. d Representative immunofluorescence staining of ASC (green) and NLRP3 (red) in primary microglia with indicated treatments. The white dotted boxes in images are magnified on the right. White arrows indicate ASC specks. e Quantification of relative fluorescent intensities of NLRP3. f Quantification of the percentages of ASC specks-positive primary microglia. gm IB analysis and quantification of NLRP3 inflammasome signals in supernatants and cell lysates of PFFs or PBS-treated primed-primary microglia cells challenged with or without ASC specks. Data are shown as representative plots g and bands quantified by densitometric analysis (hm). Data are presented as mean ± SEM for at least n = 3 and are analyzed by one-way ANOVA followed by Tukey’s post hoc test for multiple comparisons. Significance levels are indicated as: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns no significant. Scale bars are as indicated

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