Fig. 4

ASC specks enhanced reactive microgliosis. a Representative immunofluorescence staining of Iba1 (green) in ipsilateral striatum, SN and motor cortex of indicated mice brains. White dotted squares show the morphology of the microglia after magnification. b–d Quantification of the relative Iba1-positive cell numbers in ipsilateral striatum, SN and motor cortex of indicated mice brains (n = 3 or 4). e, f IB analysis and quantification of Iba1 in bilateral striatum (n = 3 or 4). g, h IB analysis and quantification of Iba1 in bilateral SN tissues (n = 3 or 4). i, j IB analysis and quantification of Iba1 in bilateral cortex (n = 3 or 4). Values are presented as mean ± SEM and are analyzed by one-way or two-way ANOVA followed by Tukey’s post hoc test for multiple comparisons. Significance levels are: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 (Right hemisphere of brain tissues); ^#p < 0.05; ^##p < 0.01; ^###p < 0.001; ^####p < 0.0001 (Left hemisphere of brain tissues). Scale bar: 100 μm. L left, R right, SN substantia nigra