Fig. 1
Time courses of corneal disease manifestations, corneal nerve density, and cornea-infiltrating and circulating myeloid cells after sterile injury to cornea. A Experimental time frame and representative corneal photographs at 1, 7 and 28 d post-injury. B Time courses of corneal epithelial defect, stromal opacity and neovascularization as graded by standardized scoring systems. n = 5 at each time-point. C, D Representative β-tubulin III staining images of corneal whole-mounts at indicated time-points post-injury (C). Amplified views of corneal center (blue box) and of corneal quadrant oriented to limbal margins at top (red box). Quantification of corneal nerve density expressed as percentage of threshold area positive for β-tubulin III signal per field of view (D). n = 8 at each time-point. E, F Representative images of Ly6G staining of corneal whole-mounts with each amplified view (yellow box) (F). Quantification of Ly6G+ cell infiltration into cornea measured as percentage of threshold area positive for Ly6G signal per field of view (E). n = 8 at each time-point. G. Representative image of Ly6C-stained corneal whole-mounts with amplified view of corneal center (red box) at 7 d post-injury. H, I Representative and quantitative flow cytometry results for CD11bhiLy6Ghi cells and CD11bhiLy6ChiLy6Glo cells in peripheral blood before injury and at 1 and 7 d post-injury. Each circle represents the data from an individual animal. Mean values + SD are shown in B. Mean values ± SD are shown in D, E and I