Fig. 3

Burrowing and Von Frey assay. Burrowing activity in sham and endometriotic mice were assessed by measuring the weight of food left inside the tube at the following timepoints: 20 min, 2 h, 3 h, 5 h, 7 h, and 24 h. A No differences in burrowing behavior were observed before day 15 (days 4–14) between sham controls and endometriotic mice. B After day 15 (days 16–30), mice with endometriosis had reduced burrowing than sham controls at all timepoints except at 24 h. Von Frey assay showed decreased C abdominal withdrawal and D Paw withdrawal threshold in endometriotic mice. Values represent mean ± standard error mean (SEM), n = 4–6 mice/timepoint. The asterisks indicate significant differences between groups, **(p < 0.01) and ***(p < 0.001)