Fig. 2

Characterization of rat circMETTL9. A Measurement of relative circMETTL9 express in multiple tissues by qPCR (brain, heart, liver, spleen, lung, and kidney, n = 3 per group) showing brain enrichment. B Sanger sequencing showing the spliced junction of circMETTL9. C Presence of circMETTL9 in rat cerebral cortex validated by RT-PCR and agarose gel electrophoresis. CircMETTL9 was amplified by divergent primers from cDNA but not from gDNA. D Expression of circMETTL9 and METTL9 in rat cortex after treatment with RNase R as measured by qPCR (n = 3 per group). Data presented as mean ± SEM, ****P < 0.0001 by Student’s t-test. E Localization of circMETTL9 in astrocytes by RNA fluorescence in situ hybridization (FISH). Nuclei were counterstained with DAPI. Scale bar = 10 µm