Fig. 3
From: Septin-3 autoimmunity in patients with paraneoplastic cerebellar ataxia
Indirect immunofluorescence analysis of single septin or septin-3/5/6/7/11 complex transfected HEK293 cells with patient serum. Acetone-fixed recombinant HEK293 cells expressing septin-3, septin-5, septin-6, septin-7 or septin-11 individually or co-expressing septin-3/5/6/7/11 or an empty vector-transfected control were incubated with the patient serum (PS1) or an anti-septin-5 or anti-septin-7 positive serum (1:100). All anti-septin positive sera showed a positive reaction with the recombinant septin-3/5/6/7/11 expressing HEK293 cells. Additionally, PS1 recognized cells expressing recombinant septin-3 but none of the other single septin expressing HEK293 cells. Recombinant septin-5 or septin-7 expressing HEK293 cells were recognized by the anti-septin-5 or anti-septin-7 positive control serum, respectively. (Scale bar 100 µm.)