Fig. 4

Neutralization of indirect immunofluorescence reaction on neuronal tissues with patient serum. Patient serum 1 (PS1, 1:100) was pre-incubated with extracts of HEK293 cells transfected with empty control vector (A) or with septin-3/5/6/7/11 (B), septin-3 (C) or septin-5 (D) before an indirect immunofluorescence assay with neuronal cryosections and Alexa Fluor 488 labeled goat anti-human IgG as secondary antibody was performed. The extract containing the septin-3/5/6/7/11 complex or septin-3 alone (B, C) abolished the immune reaction of PS1 on rat hippocampus, rat and primate cerebellum. The HEK293 control and the HEK293-septin-5 extracts (A, D) had no effect. Nuclei were counterstained with TO-PRO-3 iodide (blue). (Scale bar: 100 µm.)